[KO Validated] N-Cadherin Rabbit mAb

时间：2020-10-29 09:57 浏览次数：1417次

品牌	ABclonal	产品类型	抗体
产品名称	[KO Validated] N-Cadherin Rabbit mAb	产品规格	50ul /100ul /200ul
产品目录号	A19083	产品目录价	800/1200/2000

背景

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

免疫原信息

免疫原	A synthesized peptide derived from human N Cadherin
序列	Email for sequence
基因ID	1000
Swiss Prot	P19022
别名	CD325;CDHN;CDw325;NCAD;N Cadherin;CDH2
研究领域	Cancer癌症Invasion and Metastasis侵袭和转移 Cell Biology & Developmental Biology细胞生物学-发育生物学Cell Cycle细胞周期Centrosome中心体 Cell Biology & Developmental Biology细胞生物学-发育生物学Cell Adhesion细胞粘附Cadherins钙黏着蛋白 Cell Biology & Developmental Biology细胞生物学-发育生物学Cell Adhesion细胞粘附Tight Junctions紧密连接 Cell Biology & Developmental Biology细胞生物学-发育生物学Wnt/β-Catenin Signaling PathwayWnt/β-Catenin信号通路 Immunology & Inflammation免疫学与炎症CDsCD分子 Stem Cells干细胞Hematopoietic Progenitors造血祖细胞

应用

反应物种	Human, Mouse, Rat
验证应用	WB IHC IF
推荐稀释比&实验步骤	WB 1:500 - 1:2000 [ 实验步骤 ] IHC 1:50 - 1:200 [ 实验步骤 ] IF 1:50 - 1:200 [ 实验步骤 ]
理论分子量	140kDa
实际分子量	140kDa
存储缓冲液	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
实验方法	Western blotting
阳性样本	HeLa,293T,NIH/3T3,C6
细胞定位
纯化方式	Affinity purification

[KO Validated] N-Cadherin Rabbit mAb 检测图片

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (A19083) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A19083) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry of paraffin-embedded mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry of paraffin-embedded rat heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunofluorescence analysis of mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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