产品介绍

背景

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

免疫原信息

免疫原	A synthetic peptide of human LC3B.
序列	Email for sequence
基因ID	81631
Swiss Prot	Q9GZQ8
别名	LC3B;ATG8F;MAP1LC3B-a;MAP1A/1BLC3;MAP1LC3B;LC3A/LC3B
研究领域	Cell Biology & Developmental Biology细胞生物学-发育生物学Cytoskeleton细胞骨架Microtubules微管 Cell Biology & Developmental Biology细胞生物学-发育生物学Autophagy自噬ATGsATG家族

应用

反应物种	Human, Mouse, Rat
验证应用	WB IHC IF
用户验证的应用
推荐稀释比&实验步骤	WB 1:500 - 1:2000 [ 实验步骤 ] IHC 1:50 - 1:200 [ 实验步骤 ] IF 1:50 - 1:200 [ 实验步骤 ]
理论分子量	14kDa,16kDa
实际分子量	14kDa,16kDa
存储缓冲液	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
实验方法	Western blotting
阳性样本	HeLa,293T,C6,NIH/3T3
细胞定位	Cytoplasm,Cytoplasmic vesicle,Endomembrane system,Lipid-anchor,autophagosome,autophagosome membrane,cytoskeleton
纯化方式	Affinity purification

[KO Validated] LC3B Rabbit mAb 检测图片

Western blot - [KO Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of extracts of various cell lines, using LC3B antibody (A19665) at 1:1000 dilution.293T cells, LC3B knockout (KO) 293T cells, C6 cells and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Western blot - [KO Validated] LC3B Rabbit mAb (A19665)

Western blot analysis of extracts of Hela cells, using LC3B pAb (A19665) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry of paraffin-embedded Rat brain using LC3B antibody (A19665) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry of paraffin-embedded Human brain using LC3B antibody (A19665) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] LC3B Rabbit mAb (A19665)

Immunohistochemistry of paraffin-embedded Mouse spinal cord using LC3B antibody (A19665) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)

Immunofluorescence analysis of C6 cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)

Immunofluorescence analysis of HeLa cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] LC3B Rabbit mAb (A19665)

Immunofluorescence analysis of NIH/3T3 cells, untreated(right) and Chloroquine treated(50 μM, 37℃ for 20 hours;left) using LC3B antibody (A19665) at dilution of 1:100. Blue: DAPI for nuclear staining.

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